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Schematic illustration ( a ) and characterization of the synthetic <t>E.</t> <t>coli</t> strains Em and EmEAP1. ( a ) A scheme indicating the construction of the strains and the induction of protein exposure in the synthetic cells. ( b ) Confocal images of the Em and EmEAP1 cells after induction of IPTG for 12 h. The cells were stained by DAPI (blue fluorescence, indicating bacterial DNA) and the FITC (green fluorescence)-conjugated anti-Eap1 body for observation. The mCherry field indicates the synthetic mCh/mChEap1 expressed by the E. coli cells. ( c ) Quantification of the FITC fluorescence intensity of the stained cells. The asterisk indicates a significant difference between the two groups ( p < 0.05).
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Schematic illustration ( a ) and characterization of the synthetic <t>E.</t> <t>coli</t> strains Em and EmEAP1. ( a ) A scheme indicating the construction of the strains and the induction of protein exposure in the synthetic cells. ( b ) Confocal images of the Em and EmEAP1 cells after induction of IPTG for 12 h. The cells were stained by DAPI (blue fluorescence, indicating bacterial DNA) and the FITC (green fluorescence)-conjugated anti-Eap1 body for observation. The mCherry field indicates the synthetic mCh/mChEap1 expressed by the E. coli cells. ( c ) Quantification of the FITC fluorescence intensity of the stained cells. The asterisk indicates a significant difference between the two groups ( p < 0.05).
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ATCC chassis strain c1
Schematic illustration ( a ) and characterization of the synthetic <t>E.</t> <t>coli</t> strains Em and EmEAP1. ( a ) A scheme indicating the construction of the strains and the induction of protein exposure in the synthetic cells. ( b ) Confocal images of the Em and EmEAP1 cells after induction of IPTG for 12 h. The cells were stained by DAPI (blue fluorescence, indicating bacterial DNA) and the FITC (green fluorescence)-conjugated anti-Eap1 body for observation. The mCherry field indicates the synthetic mCh/mChEap1 expressed by the E. coli cells. ( c ) Quantification of the FITC fluorescence intensity of the stained cells. The asterisk indicates a significant difference between the two groups ( p < 0.05).
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Bacterial strains used in this study.
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ATCC chassis strain
Bacterial strains used in this study.
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Beijing Science and Technology Co Ltd model strain or chassis organism
Bacterial strains used in this study.
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Schematic illustration ( a ) and characterization of the synthetic E. coli strains Em and EmEAP1. ( a ) A scheme indicating the construction of the strains and the induction of protein exposure in the synthetic cells. ( b ) Confocal images of the Em and EmEAP1 cells after induction of IPTG for 12 h. The cells were stained by DAPI (blue fluorescence, indicating bacterial DNA) and the FITC (green fluorescence)-conjugated anti-Eap1 body for observation. The mCherry field indicates the synthetic mCh/mChEap1 expressed by the E. coli cells. ( c ) Quantification of the FITC fluorescence intensity of the stained cells. The asterisk indicates a significant difference between the two groups ( p < 0.05).

Journal: Vaccines

Article Title: Construction of Candida albicans Adhesin-Exposed Synthetic Cells for Preventing Systemic Fungal Infection

doi: 10.3390/vaccines11101521

Figure Lengend Snippet: Schematic illustration ( a ) and characterization of the synthetic E. coli strains Em and EmEAP1. ( a ) A scheme indicating the construction of the strains and the induction of protein exposure in the synthetic cells. ( b ) Confocal images of the Em and EmEAP1 cells after induction of IPTG for 12 h. The cells were stained by DAPI (blue fluorescence, indicating bacterial DNA) and the FITC (green fluorescence)-conjugated anti-Eap1 body for observation. The mCherry field indicates the synthetic mCh/mChEap1 expressed by the E. coli cells. ( c ) Quantification of the FITC fluorescence intensity of the stained cells. The asterisk indicates a significant difference between the two groups ( p < 0.05).

Article Snippet: The E. coli strain BL21 (the chassis) was obtained from GenScript (GenScript Biotech Corp., Nanjing, China).

Techniques: Staining, Fluorescence

Bacterial strains used in this study.

Journal: Biology

Article Title: l -Serine Biosensor-Controlled Fermentative Production of l -Tryptophan Derivatives by Corynebacterium glutamicum

doi: 10.3390/biology11050744

Figure Lengend Snippet: Bacterial strains used in this study.

Article Snippet: C1* , Genome-reduced chassis strain of ATCC 13032 , [ ] .

Techniques: Over Expression